...NMR Studies of Membrane Proteins - Groupe de RMN biomolé...

来自 : hal.inria.fr/IBS-RMN/hal-01571 发布时间：2021-03-24

Abstract : Methyl groups are very useful probes of structure, dynamics and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter we discuss the approach for labelling methyl groups in E. coli based protein expression, as exemplified with the mitochondrial carrier GGC.
Methyl groups are very useful probes of structure, dynamics and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide ...NMR spectroscopy ; methyl labelling ; refolding ; deuteration ; detergentMethyl groups are very useful probes of structure, dynamics and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter we discuss the approach for labelling methyl groups in E. coli based protein expression, as exemplified with the mitochondrial carrier GGC.Methyl